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Journal of Animal and Veterinary Advances
Year: 2009 | Volume: 8 | Issue: 7 | Page No.: 1256-1262
Effects of Lycopene and Vitamine E Administration Over Gastric Mucosal Damage Induced by Aflatoxin B1
Dogan Kurt , Berna Guney Saruhan , Beran Yokus and Dilek Ulker Cakir
 
Abstract: In the present study, we aimed to determine, the changes induced by Aflatoxin B1 (AFB1) administration on rat gastric mucosal barrier and gastric mucins alongside revealing the protective effects of lycopene and Vit E. Thirty-five Wistar-Albino male rats weighing 180-220 g, were divided into 7 groups as to include 5 rats in each group: control, lycopene (10 mg/kg/day lycopene for 15 days (Lycopene 10% FS), AFB1 (single dose of 2.5 mg AFB1 kg-1 on the 12th day of the study), Lycopene + AFB1 (10 mg lycopene/kg/day for 15 days and single dose of 2.5 mg AFB1 kg-1 on the 12th day of the study) and Vitamin E + AFB1 (10 mg/kg/day Vitamin E for 15 days and single dose 2.5 mg AFB1 kg-1 on 12th day). Following the sacrifice of study subjects on the 15th day, gastric mucus and phospholipid levels were determined and their stomachs were examined histopathologically. Examination of mucus and phospholipid levels revealed a significant reduction in group 3-5, in which AFB1 has been applied (respectively, p<0.001, p<0.001). When lycopene and vitamine E groups are compared with the AFB1 group, a significant elevation was detected in mucus and phospholipid levels (respectively, p<0.001, p<0.001). Whereas, histopathological examination of gastric mucosas of the aflatoxin group showed degenerative changes, gastric mucosas of the control group and the remaining study groups were normal. Histochemically, while neutral mucins were predominant in general structure of stomach, mixed and sialomucins were observed, as well. Particularly acid mucins with suplhate and periodate reactive acid mucins were found to be more predominant in the aflatoxin group compared to control and other groups. Histochemical features of mucins were observed to be consistent with specific functions of the different regions of stomach. Lycopene and vitamine E administrations were found to be protective against the damage induced by aflatoxin on gastric mucosa.
 
How to cite this article:
Dogan Kurt , Berna Guney Saruhan , Beran Yokus and Dilek Ulker Cakir , 2009. Effects of Lycopene and Vitamine E Administration Over Gastric Mucosal Damage Induced by Aflatoxin B1. Journal of Animal and Veterinary Advances, 8: 1256-1262.
URL: http://medwelljournals.com/abstract/?doi=javaa.2009.1256.1262