Abstract: The present research was conducted in order to investigate the effects of right-unilateral lesion of substantia nigra neurons by means of Ibotonic acid, a cholinergic-selective neurotoxin, on hematological parameters in rats. Thirty male Wistar rats weighing 200±50 g at the start of the experiment were used. The substantia nigra was right-unilateral lesioned by stereotaxic microinjections of 8 μg (free base) ibotonic acid, dissolved in 4 μL physiological saline containing 0.1% ascorbic acid, administered through the Hamilton microsyringe over 4.50 min. Seven days after neurosurgery, we assessed the total number of White Blood Cells (WBC), the total number of Red Blood Cells (RBC), Red cell Distribution (RDW), platelet and hemoglobin level and the erythrocyte indexes, Mean Cell Volume (MCV), Mean Cell Hemoglobin (MCH), Mean Cell Hemoglobin Concentration (MCHC). Ibotonic acid treatment induced a highly significantly decrease of white blood cells (p<0.001), followed by significant decrease in red blood cells (p = 0.02) and hemoglobin level (p = 0.03) comparative with sham-operated rats. Also, in the ibotonic acid-lesioned rats the erythrocyte indexes, Mean Cell Volume (MCV) (p = 0.04); Mean Cell Hemoglobin (MCH) (p = 0.02)) were significantly decreased comparative with sham-operated rats. By contrast, in the ibotonic acid-lesioned rat's platelet (0.34), Mean Cell Hemoglobin Concentration (MCHC) (p = 0.41) and Red blood cell Distribution Width (RDW) (p = 0.03) were significantly enhanced comparative with sham-operated rats. On the whole, the obtained data indicate the important role of the central nerves system in the regulation of erythrocyte dynamics.
Fakher Rahim, Bijan Keikhaei, Alireza Sarkaki and Abdol Hassan Doulah, 2009. Hematological Disorders in Ibotonic Acid-Induced Rat Model of Alzheimer's Disease. Journal of Animal and Veterinary Advances, 8: 2262-2268.