Abstract: Based on QTL fine mapping results on bovine chromosome 6 in the previous studies, KLF3 gene was chosen as a candidate gene to evaluate its effect on milk production traits in a Chinese Holstein population. A SNP (NC_007304:g.13849 G>C) in exon4 of KLF3 gene was demonstrated by directly sequencing of PCR product. Milk production traits including milk yield, fat yield, protein yield, fat percentage and protein percentage of each cow were collected by using 305 days lactation records. The further association analysis of the SNP genotype with milk production traits were conducted in a Chinese Holstein population including 1417 cows. We found that there were a SNP associated significantly with milk yield (p = 0.022) and protein yield (p = 0.033). The effect of CC genotype of SNP (NC_007304:g.13849 G>C) was significantly higher than other genotype (p<0.05) in both traits. The study suggested that the KLF3 gene could be used for marker assisted selection program as a molecular marker gene for milk yield, protein yield in Holstein dairy cattle breeding.
Zongjun Yin, Gui Mei, Yang Liu, Xiangdong Ding and Qin Zhang, 2010. Polymorphism Identification and Association with Milk Production Traits of KLF3 Gene in a Chinese Holstein Population. Journal of Animal and Veterinary Advances, 9: 2784-2787.