Abstract: A simple spectral classical least square calibration approach were proposed for the simultaneous quantitative evaluation and stability testing of sulphadiazine and trimethoprim in an animal commercial preparation, bolus without requiring a priory separation step. Stability tests of sulphadiazine and trimethoprim in bolus were performed by using the following conditions: Room temperature, refrigerator and etuve (37°C) for each 3 month during 12 months. A spectral classical least square calibration based on the use of absorptivities at three-wavelength set (241, 257 and 288 nm) was computed by using the linear concentration range of 4.0-22.0 μg mL-1 for sulphadiazine and 3.0-18.0 μg mL-1 for trimethoprim. The proposed spectral least square calibration was validated by using various synthetic binary mixtures containing the above veterinary drugs. Simultaneous analysis and stability test of sulphadiazine and trimethoprim in commercial veterinary bolus was established by the spectral classical calibration method.
Hismiogullari Sahver Ege, Yarsan Ender and Dinc Erdal, 2010. Spectral Classical Least Square Calibration Approach for the Simultaneous Determination and Stability Test of Sulphadiazine and Trimethoprim in Bolus. Journal of Animal and Veterinary Advances, 9: 857-861.