Abstract: Observation on morphological and ultrastructural changes of retinopathy in diabetic rabbit long been fed with high sucrose and fat fodders. Use alloxan to establish diabetic rabbit model. Feed the rabbits with high sucrose and fat fodders conduct a Fluorescein Fundus Angiography test (FFA) every 3~4 months to observe morphological changes in retina. Conduct Transmission Electron Microscopy (TEM) examination on eyeball of the model 10~12 months later so as to get wise to the ultrastructural changes in the rabbit retina. The FFA indicated that with the protraction of the disease, Fluorescein Isothiocyanate (FITC) leakage and neo-vasulariztion shadows similar to that of human were seen in the diabetic rabbit model. TEM examination showed that no abnormality was seen in the ultrastructure of the rabbit retina of normal group. The retina of the diabetic rabbit exhibited microfilament and focal dissolving, myelin sheath structure loosening and partial demyelination changes, pericyte mitochondrion tumefaction, endothelial cell layering, space increasing, mitochondrion tumefaction on the nerve fiber layer. Glycogenosome accumulation was seen in the outer nuclear layer retina cells.
Wentao Sun, ChunLing Lei, Sihai Zhao, LiLun Wang, ChunChao Bi, Rui Wang, XiaoLiang Zhou, Enqi Liu and MingXia Chen, 2011. Histomorphological and Ultrastructural Changes of Retinopathy in Diabetic Rabbit Model. Journal of Animal and Veterinary Advances, 10: 133-137.