Abstract: In the present study, the ES-like cells were derived from chicken fetal ovarian. These cells exhibit features similar to those of chicken EG cells such as typical bird nest morphology, strong reactivity toward periodic acid-Schiff and alkaline phosphatase staining. They could be maintained for more than a month on STO feeder in vitro, expressed the pluripotent markers such as stage specific embryonic antigen-1, stage-specific embryonic antigen-4, tumor rejection antigen-1-60 and tumor rejection antigen-1-81. Furthermore, the ES-like cells could form embryoid bodies and differentiate to several kinds of cells in vitro. Their easy accessibility makes these cells an attractive alternative to chicken ES cells for future functional and genomic studies and comparison with other stem cells.
Chuan-Sheng Zhang, Li-Ying Geng, Chun-Guang Yin, D.U. Li-Xin, Zheng-Zhu Liu, Zhi-Xin Fu, Shen Ping, Chen Juan, Qing-Hui Jia and Xie-Rong Liu, 2011. Derivation and Characteristics of ES-Like Cells in Chicken Fetal Ovarian. Journal of Animal and Veterinary Advances, 10: 241-245.