Authors : G.Y. Liu
Abstract: The complete CDS sequences of three goat genes-goat RAB14, RB11B and SOD3 were amplified using RT-PCR. Sequence analysis of these three genes revealed that the goat RAB14 gene encodes a protein of 215 amino acids and has high homology with the Ras-related protein Rab-14 (..RAB14) of seven species-rat and pig (100%) human, mouse, chicken and orangutan (99%), dictyostelium discoideum (71%). The goat RB1B gene encodes a protein of 218 amino acids and has high homology with the Ras-related protein Rab-11B (RB11B) of five species-human, rat and bovine (99%), mouse (98%), electric ray (97%). The goat SOD3 has high homology with the superoxide dismutase 3, extracellular (SOD3) of four species-rabbit (74%), human (74%), rat (61%) and mouse (60%). The phylogenetic tree analysis revealed that the goat RAB14 gene has a closer genetic relationship with the RAB14 gene of rat and pig and the goat RB1B gene has a closer genetic relationship with the RB11B of human, rat and bovine. The goat SOD3 gene has a closer genetic relationship with the SOD3 of rabbit. The gene expression profile analysis indicated that the goat RAB14, RB11B and SOD3 genes were differentially expressed in tissues including ovary, pituitary, muscle, kidney, heart, lung, liver and spleen. The experiment established the primary foundation for further research on these three goat genes.
G.Y. Liu , 2011. Sequence Characterization and Expression Pattern of the Capra hircus (Goat) RAB14, RB11b and SOD3 Genes. Journal of Animal and Veterinary Advances, 10: 262-268.