Journal of Animal and Veterinary Advances

Year: 2011
Volume: 10
Issue: 5
Page No. 585 - 592

Molecular Cloning and Sequence Characterization and Tissue Transcription Profile Analyses of Three Novel Common Carp (Cyprinus carpio) Genes-RaB7, RaB8a and RDH1l

Authors : Kong Lingfu and BI Baoliang

Abstract: The complete coding sequences of three common carp genes RAB7, RAB8A and RDH1L were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) based on the sequence information of the zebrafish and referenced highly homologous common carp ESTs. The sequence analyses of these three genes revealed that common carp RAB7 gene encodes a protein of 204 amino acids which has high homology with the RAB family member rab-7 (RAB7) of fifteen species: Atlantic salmon (97%), zebrafish (91%), sheep (90%), western clawed frog (89%), red jungle fowl (90%), African clawed frog (88%), human (89%), dog (89%), horse (89%), Sumatran orangutan (89%), cattle (89%), rat (89%), mouse (89%), Aiptasia pulchella (88%) and rabbit (87%). The common carp RAB8A gene encodes a protein of 207 amino acids which has high homology with the cmember RAS oncogene family rab8a (RAB8A) of eight species: zebrafish (98%), human (92%), dog (92%), cattle (92%), mouse (91%), rat (91%), Sumatran orangutan (91%) and chicken (87%). The common carp RDH1L gene encodes a protein of 319 amino acids that has high homology with tthe retinol dehydrogenase 1, like (RDH1L) of two species: zebrafish (83%) and Atlantic salmon (65%). Phylogenetic tree analysis revealed that the common carp RAB8A and RDH1L have closer genetic relationships with the zebrafish RAB8A and RDH1L but the common carp RAB7 has a closer genetic relationship with the RAB7 of Atlantic salmon. The tissue transcription profile analyses indicated that that the common carp RAB7, RAB8A and RDH1L genes are generally but differentially expressed in the detected tissues including in tissues including muscle, heart, brain, skin, gills, eye, fin. These data serve as a foundation for further research on these three genes.

How to cite this article:

Kong Lingfu and BI Baoliang, 2011. Molecular Cloning and Sequence Characterization and Tissue Transcription Profile Analyses of Three Novel Common Carp (Cyprinus carpio) Genes-RaB7, RaB8a and RDH1l. Journal of Animal and Veterinary Advances, 10: 585-592.

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