Abstract: To optimize prebiotics and growth factors for maximizing the calcium conversion of a probiotic, Lactobacillus rhamnosus during the fermentation of boving bone-meal and to assess the effects of these factors by using response surface methodology. After single factor experiment determined the addition of prebiotics and growth factors, conversion rate characteristic of calcium as indicators. Response Surface Methodology (RSM) was used to optimize the best for enhancing the prebiotics and growth factors of Lactobacillus rhamnosus for calcium conversion. It was found that prebiotics were the most effective for calcium conversion, amino acids second, vitamin minimum the last. Analyzing the response surface contour and surface plots, the optimal combination of the prebiotics and growth factors were determined as nicotinic acid/vitamin B2 = 2.82:1, arginine/met = 1.82:1, glucose/isomaltooligosaccharide = 3.80:1. The best conversion rate of calcium was 28.32%.
A.P. Luo, Y.Y. Tang, Y.Y. Yin and L.F. Wang, 2011. Optimization of Synbiotics and Growth Factors for Calcium Conversion of Lactobacillus rhamnosus Based on Response Surface Methodology. Journal of Animal and Veterinary Advances, 10: 993-998.