Authors : Liu Lixian, Wang Pei , Huo Jinlong , Huo Hailong , Zhao Yue , Wang Rui and Zeng Yangzhi
Abstract: The complete CDS sequence of Banna Mini-pig Inbred line (BMI) gene CENPP was amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to Accession No.: JF944894. Sequence analysis revealed that the BMI CENPP encodes a protein of 291 amino acids that has high homology with the Centromere Protein P (CENPP ) of seven other species-horse (85%), cattle (83%), human (77%), orangutan (76%), monkey (76%), mouse (70%) and rat (68%). The phylogenetic tree analysis revealed BMI CENPP has a closer genetic relationship with the horse CENPP than with those of cattle, human, orangutan, monkey, mouse and rat. Analysis by RT-PCR showed that BMI CENPP gene was over-expressed in midbrain, ovary, spleen, lung, skin, muscle, moderately expressed in diencephalon, heart, nerve fiber, stomach, small intestine, large intestine and almost not expressed in other 6 tissues. Two microRNA target sites were predicted in the CDS of BMI CENPP mRNA for further studying this gene in the future. The experiment will establish a foundation for further insight into this swine gene.
Liu Lixian, Wang Pei , Huo Jinlong , Huo Hailong , Zhao Yue , Wang Rui and Zeng Yangzhi , 2012. Identification, mRNA Expression and Characterization of a Novel CENPP Gene from Chinese Banna Mini-Pig Inbred Line (BMI). Journal of Animal and Veterinary Advances, 11: 1230-1236.