Authors : Uyunbilig Borjigin, Ting Yun , Muren Herrid and Shorgan Bou
Abstract: Identification and isolation of Spermatogonial Stem Cells (SSCs) are a prerequisite for culture, genetic manipulation and transplantation research. In this study, the appropriate age of the donor ram for SSCs isolation was investigated. Spermatogonial stem cell specific marker protein Promyelocytic Leukaemia Zinc-Finger (PLZF) and male germ cell marker VASA double staining were checked on the paraffin sections of ovine testis. Type A spermatogonial marker PGP9.5 and sertoli cell marker Vimentin double staining were also used to confirm testes development. Results indicated that testes from neonatal rams had only one type of undifferentiated germ cell gonocyte and should be suitable for ovine SSCs isolation. VASA and Vimentin double staining of the isolated testis cells confirm the efficiency of ovine SSCs isolation.
Uyunbilig Borjigin, Ting Yun , Muren Herrid and Shorgan Bou , 2012. Characterization and Isolation of Ovine Spermatogonial Stem Cells. Journal of Animal and Veterinary Advances, 11: 1242-1245.