Authors : Jinlong Huo, Pei Wang, Hongjiang Wei and Yangzhi Zeng
Abstract: The complete coding sequence of Banna Mini-pig Inbred line (BMI) gene PQBP1 was amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to accession number JF750401. Sequence analysis revealed that the BMI PQBP1 encodes a protein of 265 amino acids that has high homology with the Polyglutamine Binding Protein1 (PQBP1) of five other species-human (95%), monkey (95%), cattle (95%), mouse (88%) and rat (87%). The phylogenetic tree analysis revealed BMI PQBP1 has a closer genetic relationship with the cattle PQBP1 than with those of human, monkey, mouse and rat. Analysis by RT-PCR showed that BMI PQBP1 gene was over-expressed in midbrain, ovary, spleen, lung, nerve fiber, skin and muscle, moderately expressed in diencephalon, kidney, heart, stomach, small intestine, large intestine and fat, weakly expressed in lymph node and cerebrum and almost not expressed in liver and pancreas. One microRNA target site was predicted in the CDS of BMI PQBP1 mRNA for further studying this gene in the future. These data serve as a foundation for further insight into this swine gene.
Jinlong Huo, Pei Wang, Hongjiang Wei and Yangzhi Zeng, 2012. Molecular Cloning, Sequence Characterization and Tissue Transcription Profile of a Novel Gene PQBP1 from Chinese Banna Mini-Pig Inbred Line (BMI). Journal of Animal and Veterinary Advances, 11: 1674-1680.