Authors : Liu Yong, Ding Biao, Yang Xunxun, Shang Mingbao, Lei Xuehua, Wang Rong, Wu Fengrui and Li Wenyong
Abstract: Oocytes grow and obtain the ability of resume meiosis and maturation during follicular growth. Many regulatory mechanisms play a role in this process. DNA methylation and chromatin configuration are important regulatory mechanisms of gene transformation. The dynamic transformation of DNA methylation and chromatin configuration should be researched during follicular growth. Laser 3D sequence scanning and quantitative analysis fluorescence were used to discover the intensity and position of DNA methylation in porcine oocytes from different diameter follicles. The total fluorescence intensity (9374±2886 vs. 2703±473) and area (40914±7264 vs. 18862±2216) reduced during the follicular growth but the average fluorescence intensity (0.22±0.03 vs. 0.17±0.16) had no significant difference in <1 and >3 mm group although reduced in 1-3 mm group (0.13±0.04). Confocal microscopy was also used to compare the relationship between DNA methylation and chromatin configuration. The position of DNA methylation matched chromatin configuration well in most oocytes but researchers found some different situations that need to be further researched.
Liu Yong, Ding Biao, Yang Xunxun, Shang Mingbao, Lei Xuehua, Wang Rong, Wu Fengrui and Li Wenyong, 2012. Dynamic Transformation of DNA Methylation and Chromatin Configuration in Porcine Oocyte during Follicular Growth. Journal of Animal and Veterinary Advances, 11: 1739-1744.