Abstract: Molecular diagnostics methods are used to investigate mycoplasma infection status but this approach to infection analysis of mycoplasma in endometritis of dairy cow has not been reported. In this study, a nest Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis (nested PCR-DGGE) assay for detection of 16S ribosomal RNA of mycoplasma was used as reference of 4 isolates which were identified in a previous survey of dairy cows reproductive system in Jilin province, China. A reference PCR-DGGE profile was generated using DNA extracted and amplified from the strain of these cultures. The effectiveness of the procedure was assessed by application to test the samples from endometritis dairy cows uterus mucus. The bands from the nested PCR-DGGE were extracted for sequencing.
Meng-Yao Guo, Guo-Qing Wang, Guoxi Shao, Wulin Tian, Yuanjing Xu and Guanghong Xie, 2012. Identification of Intrauterine Mycoplasma in Endometritis of Dairy Cow Isolates by 16S rDNA Nested PCR-DGGE. Journal of Animal and Veterinary Advances, 11: 1745-1752.