Authors : Wang Qing-Rong, Wei Zhi-Qin and Song Pei-Yong
Abstract: The 600 bp fragments of 16SrRNA gene were obtained by PCR Method from the mitochondrial DNA of Silurus asotus from four species groups and a mitochondrial DNA 16SrRNA of 540 bp sequence was successfully sequenced by Clustal X nomologous sequence analysis. The three upper branch groups are from Yalong Jiang in Sichuan province, Min river in Sichuan province and Wujiang river in Guizhou province, respectively. The middle branch group is from Wuyang river in Guizhou province. The genetic identity from 1~0.9980 among groups and the genetic distance from 0~0.0020 among groups were calculated by PopGen32. The results showed that there was no variation on the gene of the species groups from Wujiang river in Guizhou province. There were some variations on the gene of Yalong Jiang in Sichuan province, Min river in Sichuan province and Wuyang river in Guizhou province. The 4 individuality there were 4 haplotypes in Min river in Sichuan province. The 4 individuality there were 4 haplotypes in Yalong Jiang in Sichuan province. The 4 individuality only 1 haplotype in Wujiang river in Guizhou province. The 6 individuality there were 2 haplotypes in the middle branch group is from Wuyang river in Guizhou province. But there were some variations among the four species groups. The dendrogram of genetic relationship constructed by PopGen32 indicates that they are divided into four branches.
Wang Qing-Rong, Wei Zhi-Qin and Song Pei-Yong, 2012. Genetic Diversity of Silurus asotus on Mitochondrial DNA 16SrRNA Gene Partial Sequence. Journal of Animal and Veterinary Advances, 11: 1843-1846.