Authors : Yan Wang, Hao Qu, Cheng-Long Luo, Jie Wang and Ding-Ming Shu
Abstract: Large deletion or mutation at the exon 10 and 3' untranslated region of Growth Factor Receptor (GHR) gene results in dwarf chicken and the common method for detecting mutation is direct PCR amplification. But since, the target fragment is large, the amplification is inefficient and unreliable and this process consumes a lot of expensive specific DNA polymerases. Researchers focused on the point mutation C146280A in the deletion fragment and set up a new method to detect large deletion and point mutation in this region. Comparing with direct PCR this new method which can detect both large deletion and point mutation with regular low-cost DNA polymerases is more stable, reliably and cheapness.
Yan Wang, Hao Qu, Cheng-Long Luo, Jie Wang and Ding-Ming Shu, 2012. A Rapid and Cost Effective Method for Genotyping Two Types of GHR Mutations in Sex-Linked Dwarf Chicken. Journal of Animal and Veterinary Advances, 11: 1873-1875.