Authors : Wang Baolin, Yuan Tao, Liu Menggang, Chen Hongxu and Chen Ping
Abstract: The hepatocellular gene transfection in vitro has been repeatedly reported but the liver gene transfection in vivo is still poorly understood. The liver gene transfection can be performed through injection via tail vein and portal vein but these methods result easily bleeding or stress reaction to the animal which may potentially confound experimental results. Here to exploring a reasonable and effective injection method for liver gene transfection, recombinant lentiviral vectors with Green Fluorescent Protein gene (GFP) were injected, respectively via the rat’s cecal vein, portal vein and tail vein and then it was observed that the GFP expression of the liver tissue and the effect of various injections on the rats. The results showed that via the cecal vein injection had the high transfection efficiency and little increase of portal vein press and the minimum effect on liver transaminase and serum IL-6 and TNF-α (n = 20) (p<0.001). It was demonstrated that in vivo liver gene transfection via the cecal vein injection in rats is a novel and reasonable method.
Wang Baolin, Yuan Tao, Liu Menggang, Chen Hongxu and Chen Ping, 2012. A Novel and Reasonable Approach for in vivo Liver Gene Transfection in Rats. Journal of Animal and Veterinary Advances, 11: 218-223.