Authors : A.A. Laith, M. Najiah, S.M. Zain, S.H.M. Effendy A.W., T. Sifzizul T.M., M. Nadirah and M. Habsah
Abstract: The objective of this study was to conduct in vitro study of antimicrobial properties of mangrove plants Sonneratia caseolaris and Rhizophora apiculata against fish pathogenic bacteria. Leaves of mangrove plant were collected from area nearby University Malaysia Terengganu. Methanol extracts of mangrove plants were obtained by using Soxhlet extractor. The bacterial cultures used were Klebsiella pneumoniae, Shigella dysenteriae, Enterobacter cloacae, Enterobacter sakazakii, Enterobacter brevis, Chryseobacterium indologenes, Stenotrophomonas maltophilia and Aeromonas hydrophila isolated from fish. The antimicrobial susceptibility test showed that the bacteria were 87.5% resistant towards colistin sulphate and 62.5% sensitive to fosfomycin. The Minimum Inhibitory Concentration (MIC) of Sonneratia caseolaris and Rhizophora apiculata ranges from 1.56-6.25 and 12.5-25 mg mL-1, respectively. The lowest Minimum Bactericidal Concentration (MBC) value was 12.5 mg mL-1 of Sonneratia caseolaris and 25 mg mL-1 of Rhizophora apiculata. The highest activity with LC50 of Sonneratia caseolaris was 6.16 mg mL-1 and Rhizophora apiculata was 0.76 mg mL-1, respectively. Methanol extracts of Sonneratia caseolaris was effective on all gram negative bacteria (Klebsiella pneumoniae, Shigella dysenteriae, Enterobacter cloacae, Enterobacter sakazakii, Enterobacter brevis, Chryseobacterium indologenes, Stenotrophomonas maltophilia and Aeromonas hydrophila) when compared to Rhizophora apiculata.
A.A. Laith, M. Najiah, S.M. Zain, S.H.M. Effendy A.W., T. Sifzizul T.M., M. Nadirah and M. Habsah, 2012. Antimicrobial Activities of Selected Mangrove Plants on Fish Pathogenic Bacteria. Journal of Animal and Veterinary Advances, 11: 234-240.