Journal of Animal and Veterinary Advances

Year: 2012
Volume: 11
Issue: 17
Page No. 3036 - 3044

Preparing Rat Spermatogonial Transplantation Recipient by Treating the Pregnant Female Rats with Busulfan

Authors : Boyang Yu, Fenhua Luo, Taodi Liu, Daiyan Liu, Linhong Liu, Yan Zhang and Yingji Wu

Abstract: Successful spermatogonial stem cells transplantation is largely dependent on the preparation of the recipient. In the present study, to acquire more efficient rat recipients, the response of pregnant female rats to treatment with 7.5-12.5 mg busulfan per kg of body weight was determined in terms of the birth rate, survival rate, body weight, testicular mass, histology, expression of certain germ cell genes as measured by real time PCR and fertility rate of male offspring. The analysis suggested that a dosage of 10 mg busulfan per kg administered to pregnant female rats at 13.5 day post pregnancy effectively prepares male pups to be recipients because it leads to the maximal deletion of endogenous spermatogonial stem cells with minimal effects on the recipientsí health.

How to cite this article:

Boyang Yu, Fenhua Luo, Taodi Liu, Daiyan Liu, Linhong Liu, Yan Zhang and Yingji Wu, 2012. Preparing Rat Spermatogonial Transplantation Recipient by Treating the Pregnant Female Rats with Busulfan. Journal of Animal and Veterinary Advances, 11: 3036-3044.

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