Journal of Animal and Veterinary Advances

Year: 2012
Volume: 11
Issue: 17
Page No. 3064 - 3067

Evaluation of the Suitability of Five Housekeeping Genes as Internal Control for Quantitative Real-Time RT-PCR Assays in Chickens Acutely Infected with IBV M41 Strain

Authors : Wen-Qiao Fan, Hong-Ning Wang, Yi Zhang and An-Yun Zhang

Abstract: Avian Infectious Bronchitis (IB) is an acute and highly contagious respiratory disease in chickens. To detect the virus in chickens, housekeeping genes were detected by quantitative real-time PCR for the normalization. The aim of study was to identify the most stable housekeeping gene (s) in IBV infection. Five housekeeping genes including 18S rRNA, β-actin, Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), Glucose-6-Phosphate Dehydrogenase (G6PDH) and Ubiquitin (UB) were investigated in nine tissues: trachea, thymus, liver, spleen, lungs, kidney, pancreas, proventriculus and bursa of Fabricius. The geNorm and Normfinder Software were used to calculate the stability of the candidate genes.

How to cite this article:

Wen-Qiao Fan, Hong-Ning Wang, Yi Zhang and An-Yun Zhang, 2012. Evaluation of the Suitability of Five Housekeeping Genes as Internal Control for Quantitative Real-Time RT-PCR Assays in Chickens Acutely Infected with IBV M41 Strain. Journal of Animal and Veterinary Advances, 11: 3064-3067.

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