Abstract: This study examined the recombinant Attenuated Salmonella Choleraesuis C500 containing M and N gene of PEDV whether can induce a systemic immune responses in mice. After identified by enzyme analysis and nucleotide sequencing test, the constructed recombinant plasmid, designated as pIRES-M-N was transfected into Vero cells. The transient expression protein was detected by indirect Immunofluorescence Assay (IFA) and Western-blotting. Result showed that pIRES-M-N can be expressed correctly in Vero cells. Then, the recombinant plasmid electroporated into S. choleraesuis C500 and the recombinant bacteria was proved to be stabile in vitro. The immunogenicities of this recombinant attenuated salmonella constructs were firstly investigated in a mouse mode. Specific anti-PEDV IgG antibodies and IFN-γ, IL-4 of cytokine were detected. The result showed that the recombinant Attenuated Salmonella could induce higher persistence specific hummune responses in mouse by contrast with naked DNA group after 42 days (p<0.05). It also, stimulated a balanced Th1/Th2 immune response. That provided the clinical basic data and theoretical basis for success of the attenuated salmonella vaccine development.
Yu-Peng Ren, Xiao-Yu Wang, Qi-Gui Yan, Di-Shi Chen, Yu Cheng, Li Wan, Lei Ma, Qi Zhang, Lin Guo, Zhi-Yong Xie and Wan-Zhu Guo, 2012. Immunogenicity of Recombinant Attenuated Salmonella Choleraesuis C500 Strain Co-Expressing M and N Protein of Porcine Epidemic Diarrhea Virus (PEDV). Journal of Animal and Veterinary Advances, 11: 3234-3241.