Authors : Meltem Boyacioglu, Hatice Parlak, Ozlem Cakal Arslan, Selma Katalay and Muhammet Ali Karaslan
Abstract: The present study aimed to assess mutagenicity of the sediment samples from Nif brook using solvent extraction method and to compare its results with those of a previous study on mutagenicity using Soxhlet-extraction method. Sediment samples were collected from 5 sites located in Nif brook in April, 2007. Sediment samples from 5 sites on Nif brook were studied for their mutagenic potential on TA 98 and 100 strains of Salmonella typhimurium using Ames test (Plate incorporation assay) without metabolic activation. Extracts of the sediment samples were assayed in three different concentrations (In 25, 50, 100 μg). Sediment sample from Nif 5 sides was observed to be weak-mutagenic and those from Nif 1 and 4 sides were observed to have toxic effects. Toxic effects of the sediment samples on bacteria were observed on the basis of significantly reduced numbers of revertants compared to the solvent controls. According to Ames criteria, sediment samples from the Nif 5 were considered as mutagenic on TA 98 strain. According to the present study, mutagenicity was detected in Nif brook by solvent extraction method.
Meltem Boyacioglu, Hatice Parlak, Ozlem Cakal Arslan, Selma Katalay and Muhammet Ali Karaslan, 2012. Mutagenicity of Sediment Samples from Nif Brook (Western Turkey). Journal of Animal and Veterinary Advances, 11: 83-87.