Authors : Rui-Ai Chen, Bing Li, Xianhao Zhang, Haopeng Liu and Dongsheng He
Abstract: Seventy four pathogenic swine Escherichia coli strains were isolated from large-scale pig farms in 5 provinces in Southern China. Fourty seven kinds of single-factor O-antigen serum were used to classify the serotypes of isolates. The serotypes of 66 of 74 swine Escherichia coli strains had been identified which belonged to 14 serotypes. O107, O101, O9, O26 and O60 were the dominant serotypes. There were 8 strains failed to be identified, accounting for 10.8% of the total strains. Eighteen swine Escherichia coli strains were selected from the dominant serotypes to pathogenicity test in mice. The results showed that 7 of 18 representative strains were virulent strains. Further, researchers analyzed the Outer Membrane Protein (OMP) of 7 virulent strains, the result showed that OMP of 7 strains, respectively from 1-3 protein bands which belonged to 3 OMP type (I~III). This is first time in Southern China to investigate systematically on the predominant serotypes of pathogenic swine Escherichia coli and laid a foundation on the prevention and control of porcine colibacillosis as well as the research of new OMP subunit vaccine.
Rui-Ai Chen, Bing Li, Xianhao Zhang, Haopeng Liu and Dongsheng He, 2013. Prevalence and Characterization of Serotype and out Membrane Protein Diversity of Escherichia coli Isolated from Southern China. Journal of Animal and Veterinary Advances, 12: 1233-1236.