Authors : Nian Fuzhao, Zhang Yonghui, Luo Dingqi, Xia Jianhua, Jiang Sheng and Zhao Leifeng
Abstract: Nicotinamide adenine dinucleotide is a drug which is important to the health of animals and humans. Nicotinamide adenine dinucleotide transporter 1 gene have been characterized to be required for nicotinamide adenine dinucleotide bioactivety. In present experiment, the complete mRNA sequence of tobacco nicotinamide adenine dinucleotide transporter 1 gene was amplified using the rapid amplification of cDNA Ends Methods. The full-length tobacco nicotinamide adenine dinucleotide transporter 1 gene mRNA was 1,588 bp containing an 948 bp open reading frame which encodes a protein of 315 amino acids. BLAST analysis revealed that tobacco nicotinamide adenine dinucleotide transporter 1 protein shares high homology with the nicotinamide adenine dinucleotide transporter 1 of Lycopersicon esculentum (93%), wine grape (86%), soybean (81%), chickpea (79%) and foxtail millet (78%). Results also showed that tobacco nicotinamide adenine dinucleotide transporter 1 gene has a closer genetic relationship with the nicotinamide adenine dinucleotide transporter 1 gene of Lycopersicon esculentum. Prediction of transmembrane helices showed that tobacco nicotinamide adenine dinucleotide transporter 1 might be a transmembrane protein. The expression profile was studied and the results indicated that tobacco nicotinamide adenine dinucleotide transporter 1 gene was highly expressed in leaf. These results established the primary foundation of using tobacco nicotinamide adenine dinucleotide as drug for animals and humans in the future.
Nian Fuzhao, Zhang Yonghui, Luo Dingqi, Xia Jianhua, Jiang Sheng and Zhao Leifeng, 2013. Molecular Characterization and Expression Pattern of a Novel Nicotinamide Adenine Dinucleotide Transporter Gene. Journal of Animal and Veterinary Advances, 12: 1271-1275.