Authors : Nian Fuzhao, Zhang Yonghui, Luo Dingqi, Xia Jianhua, Jiang Sheng and Zhao Leifeng
Abstract: Nicotinamide adenine dinucleotide have significant health benefits for animals and humans. Peroxisomal nicotinamide adenine dinucleotide carrier gene functions in mediating the nicotinamide adenine dinucleotide import into peroxisomes. The complete coding sequence of tobacco peroxisomal nicotinamide adenine dinucleotide carrier gene was amplified by RT-PCR. The open reading frame of tobacco peroxisomal nicotinamide adenine dinucleotide carrier gene was 1017 bp which encodes a protein of 338 amino acids. Sequence analysis revealed that the peroxisomal nicotinamide adenine dinucleotide carrier of tobacco shares high homology with the peroxisomal nicotinamide adenine dinucleotide carrier of Lycopersicon esculentum (88%), Fragaria vesca subsp. vesca (74%), thale cress (73%), chickpea (73%) and foxtail millet (67%). Phylogenetic tree analysis revealed that the tobacco peroxisomal nicotinamide adenine dinucleotide carrier gene has a closer genetic relationship with that of Lycopersicon esculentum. Prediction of transmembrane helices showed that tobacco peroxisomal nicotinamide adenine dinucleotide carrier might be a transmembrane protein. Expression profile was studied and the results indicated that tobacco peroxisomal nicotinamide adenine dinucleotide carrier gene was highly expressed in stem, leaf and flower. These results established the primary foundation of using tobacco nicotinamide adenine dinucleotide as drug for animals and humans in the future.
Nian Fuzhao, Zhang Yonghui, Luo Dingqi, Xia Jianhua, Jiang Sheng and Zhao Leifeng, 2013. Molecular Characterization and Expression Pattern of a Novel Peroxisomal Nicotinamide Adenine Dinucleotide Carrier Gene. Journal of Animal and Veterinary Advances, 12: 1276-1280.