Authors : Guo Wei, Ling Xiao-Qian, Hua Wen-Yu and Wang Jin-Ke
Abstract: The objective of this study was to clone Growth Hormone (GH), Insulin-like Growth Factor-I (IGF-I) and Somatostatin (SST) genes of Anhui white goat for transgenic application. The total RNAs of Anhui white goat was extracted and the target genes GH, IGF-I and SST were amplified with PCR technique, then the cDNA fragments were cloned into the eukaryotic expression vector pEGFP-N1 to construct transgenic vectors. The recombinant plasmids were transfected into the fibroblasts of Anhui white goat by liposome. The expression of the transferred GH, IGF-I and SST genes in the transgenic fibroblasts was detected by Reverse Transcription Polymerase Chain Reaction (RT-PCR). The stable transfection clones were screened by G418 resistance and detected by PCR amplification. The three genes were successfully cloned, their transgenic expression vectors were constructed and their stable transfection fibroblast clones were obtained. The constructed transgenic expression vectors and stable transfection fibroblast clones of these genes provide alternative materials for breeding transgenic goats, especially Anhui white goat.
Guo Wei, Ling Xiao-Qian, Hua Wen-Yu and Wang Jin-Ke, 2013. Cloning of GH, IGF-I and SST Genes of Anhui White Goat for Transgenic Application. Journal of Animal and Veterinary Advances, 12: 295-302.