Abstract: In this study, E. angustifolia L. was collected from different localities of Mugla in Turkey. Antibacterial and antioxidant activities of E. angustifolia L. have not been reported to the present day. The aim of the present study was to investigate the in vitro antibacterial and antioxidant activity of methanol extracts of E. angustifolia leaves. Methanol extracts were screened for antibacterial activity against mastitis pathogens. These bacteria include 2 Staphylococcus aureus and 5 Coagulase Negative Staphylococci. Antibacterial activities of the extracts were determined by the Disc Diffusion Method. Also ampicillin disc was used for comparison of inhibition zones. Methanolic leaf extract showed a maximum zone of inhibition in Staphylococcus aureus-17 by Disc Diffusion Method. E. angustifolia leaf extract showed lowest antibacterial activity against S. aureus-18 and Coagulase Negative Staphylococci-36 (CNS). The antioxidant activity of the leaf extract was also determined by ABTS Method using trolox as standard. The value found in ABTS Method is highly effective (84%). As a result, the in vitro studies clearly indicate that the methanolic leaf extract of E. angustifolia significant antioxidant activity and also a better source of natural antioxidant which might be helpful in preventing the process of different oxidative stress. In addition to, the E. angustifolia could be used in treating disease caused by the test bacteria. E. angustifolia methanolic leaf extracts have antioxidant and antibacterial potential.