Abstract: Substance P (SP) is known to play an important role in bone mineralization. However, little is known about the potential of substance P as an osteogenic inducer in human Adipose-Derived Stem Cells (hADSCs) in vitro. Therefore, the aim of this study to investigate the effects of substance P on proliferation and osteogenic differentiation of hADSCs in vitro. Human Adipose-Derived Stem Cells (hADSCs) derived from wasted adipose tissue in liposuction operation were cultured. Expression of the SP receptor (NK1) was detected by RT-PCR. Effects of SP on proliferation and differentiation of hADSCs were studied, moreover alkaline phosphatase activity was also assayed. It was found that the NK1 receptor was expressed in hADSCs. SP stimulated the proliferation of hADSCs in a concentration-dependent manner. Higher concentrations (10G8 M) of SP increased alkaline phosphatase activity and enhanced differentiated in hADSCs. NK1 receptors are expressed by hADSCs and SP stimulates osteoblast differentiation and function in vitro. These findings strengthen the knowledge about the role of SP in hADSCs.
Ming Qian, Min Liu, Mengna Duan, Haiyang Zhang, Zhe Wu and Yanmin Zhou, 2013. Substance P Induce Osteogenic Differentiation in Human Adipose-Derived Stem Cells. Journal of Animal and Veterinary Advances, 12: 863-867.