Authors : Mahmoud R. Abd Ellah, Keiji Okada, Shinsuke Shimamura, Saori Kobayashi, Reeko Sato and Jun Yasuda
Abstract: The present study, researchers evaluated for the first time serum and salivary 8-Hydroxy-2'-deoxyguanosine (8-OHdG) and salivary Total Antioxidant Capacity (TAC) during different stages of lactation and also during the dry period in dairy cows. Cows at dairy farms that belong to Morioka City (Iwate, Japan) were classified into five groups according to the month of lactation starting from postpartum till the dry period. Oxidative DNA damage marker (8-OHdG) and TAC were measured in saliva and serum samples using commercial ELISA kits. The results revealed that salivary 8-OHdG levels were higher (p<0.01) than its corresponding serum levels during both lactation and dry period. Serum levels of TAC were significantly higher (p<0.01) than their salivary levels during lactation and dry period. It could be concluded that oxidative DNA damage was prominent during the dry period. Cows at the dry period have the highest serum level of 8-OHdG and the lowest salivary level of TAC when compared to lactating cows.
Mahmoud R. Abd Ellah, Keiji Okada, Shinsuke Shimamura, Saori Kobayashi, Reeko Sato and Jun Yasuda, 2014. Status of Oxidative DNA Damage in Serum and Saliva of Dairy Cows During Lactation and Dry Period. Journal of Animal and Veterinary Advances, 13: 577-581.