Authors : Wolong Ma, Fei Guo, Antao Wang, Zhanhui Song and Chuangfu Chen
Abstract: A rifampin-resistant mutant of Brucella abortus, designated as RB51 was derived by repeated passage of strain 2308 on Trypticase soy supplemented with 1.5% agar and varying concentrations rifampin or penicillin. In this study, 325 Mb data was produced for RB51 using Illumina HiSeq 2000 sequencing platform. Based on the assemble result of RB51, researchers obtained that 36 scaffolds with GC content of 57.25% which contains 150 contigs and has genome size was about 3.26 Mb. Detail bioinformatics annotation of RB51 revealed that the genome contained 3,283 genes, the total length of genes was 2,797,347 bp which makes up 85.91% of genome. Comparative genomics analysis found that there was a tandem repeat sequence of 339 nucleotide in YP_413569.1 protein. In addition, RB51 encoded a glycosyltransferase wboA in scaffold12, an enzyme essential in the biosynthesis of O antigen which has been disrupted by IS711. In comparison with the reference genome B. abortus 2308, researchers found that RB51 contained 135 SNPs and 5 InDels among which 1 SNP and 2 InDels may be associated with virulent related to lipopolysaccharide.
Wolong Ma, Fei Guo, Antao Wang, Zhanhui Song and Chuangfu Chen, 2014. Complete Genome Sequence of B. abortus RB51 and Genomic Comparison with B. abortus 2308. Journal of Animal and Veterinary Advances, 13: 582-588.