Authors : Jian Sun, Hong-Yu Zhao, Xu-Ping Zhou and Yan Lu
Abstract: This research studies the characteristics of multidrug-resistant Salmonella from chicken. The Salmonella collected from parts of Shandong Province, serotype was measured by the method of Kauffmann-White, researchers using the Microdilution Method test the sensitivity of the isolates to 16 antimicrobial agents, the 12 resistance genes were detected by PCR, the relationship was analyzed between resistant phenotype and gene type. The results showed that 60 strains of Salmonella Indiana were isolated from 80 isolates of Salmonella. Drug susceptibility testing indicated that isolated bacterial strains to amoxicillin-clavulanic acid, cefazolin, polymyxin, ampicillin, doxycyclinea and trimethoprim 16 antimicrobial drugs resistance. The 88.33% isolates were resistant to 12-15 antimicrobial agents not found isolates of <3 antimicrobial agents. The 8 resistance genes were characterized by PCR that with int1, blaTEM, aac(6')-Ib-cr, floR, catA1, tetA, strA and cmlA. More than 90% strains carrying resistance gene int1, blaTEM, floR and aac(6')-Ib-cr. These result indicated that resistance genes were relevant to the resistant type of antimicrobial agents.
Jian Sun, Hong-Yu Zhao, Xu-Ping Zhou and Yan Lu, 2014. Characterization of Multi-Drug Resistance in Salmonella Indiana from Chicken in China. Journal of Animal and Veterinary Advances, 13: 589-593.