Abstract: The microbiological and physico-chemical analyses were carried out for fifteen boreholes in World Bank Housing Estate, Umuahia. The microbiological analysis was carried out using the Most Probable Number Technique (Multiple-Tube Technique) for the detection of faecal coliform and subsequently organisms present in the samples of water were identified following the Standard Methods. The identified organisms include: Escherichia coli, Staphylococcus sp., Streptococcus sp., Citrobacter sp., Enterobacter sp., Klebsiella pneumonia, Proteus, Pseudomonas, Serratia marcescene, 63.6% being of the family Enterobacteriaceae. Two yeast genera were identified namely: Saccharomyces and Kluyveromyces. The Total Heterotrophic Plate Count (THPC) gave a range of 1.1�10⁴-2.03�10⁵CFU mL ¹ with a mean of 5.2�10⁴CFU mL ¹ while the Total Coliform Plate Count (TCPC) gave a range of 1.1�10³-8.5�10⁵CFU mL ¹ with a mean of 4.5�10³CFU mL ¹. The Yeast Plate Count (YPC) ranged between 2.1�10¹-6.2�10¹CFU mL ¹ and a mean of 4.6�10¹CFU mL ¹. The presumptive faecal coliform ranged between 0-180 coliforms 100 mL ¹ giving a mean of 18.73 coliforms per 100 mL. E. coli/faecal coliforms were detected in 60% of the water samples. The aesthetic properties of the water samples were acceptable however the boreholes showed high values with ranges of 221-280, 52-77, 209-301 mg L ¹, 200-250 us cm ¹ for sodium, nitrate, chloride and conductivity respectively in comparison with local and international standards. These results showed that there is need for treatment of these water boreholes by the borehole proprietors and also by simple treatment methods such as boiling and agitation by the consumers.