Abstract: Water quality is closely linked to vulnerability of water sources to pollution potentials and the level of treatment processes involved. The study examined the assessment of water quality and supply of Ede Water Supply Network and assessed the physico-chemical and bacteriological quality of the water by comparing it to the existing national and international guidelines and standards for potable water. This was with a view to providing some necessary baseline data for assessing public water supply quality. Water samples were collected from 9 sampling stations (3 on the dam point, 3 on the treated point and 3 on the tap point along the pipe network). The samples were analyzed for general physico-chemical parameters (temperature, colour, conductivity, total suspended solid, total solid, turbidity, total alkalinity, total acidity, pH, total hardness), major ions (Cd, Pb, Na, K,Cu, NO₃¯, PO₄³¯, and SO₄²¯), oxygen parameters: BOD and COD and biological parameters (total coliform, E. coli, faecal coliform, total heterotrophic bacteria and total heterotrophic fungi) using standard analytical methods. The results showed that most of the investigated physical parameters and major ions were all significantly higher in dam point than treated and tap points. Most of the parameters were generally higher at the treated point than at the tap point. The concentrations of most of the investigated ions in the water samples from Ede Water Supply Network were within the permissible limits of the World Health Organization (WHO) and Nigerian Industrial Standards drinking water quality guidelines. The results showed that biological parameters of the treated and tap points were within limits of the World Health Organization (WHO) and Nigerian Industrial Standard but was higher at the dam point. In conclusion, the water quality of the Ede Water Supply Network is within to the World Health Organization guidelines and standards for drinking water supply and the government needs to do more in the aspect of adequate strengthening of the water supply distribution appurtenances to the consumers.