Authors : Farnoush Farjam, Mitra Fakhar Khorasgani, Mojtaba Kiany Boroujeni, Hooriyeh Ranjbaran, Tayebeh Abdpoursogh, Navid Farahmandian and Fateme Sefid
Abstract: Pseudomonas aeruginosa is an important cause of nosocomial infection and may lead to septicemia and death. P. aeruginosa septicaemia is associated with the highest mortality rate of all gram-negative infections. Because of the general resistance of the organism to antibiotics, research has been focused on immunotherapy. There are several bacterial cell components incorporated into subunit vaccines. Vaccine studies have often focussed on Lipo Poly Saccharide (LPS) and the outer membrane proteins (OPRs) due to its potent stimulation of the immune response. The pathophysiological nature of LPS and its serotype-specific immunological activities has limited LPS using for Pseudomona infection control whereas using major Outer Membrane Proteins of cell walls (mOMPs) of Pseudomonas aeruginosa and other gram-negative bacteria, not only is non-toxic and actively stimulate the immune system but also shows immunological cross-reactivity with mOMPs of other serotypes belonging to same species. Today, the protection of mOMPs in many pathogenic gram-negative bacteria against the corresponding etiologic factors have completely been approved. The major OPRs, OprF, OprL and OprI interested in the potential of OPRs as vaccines. Determination of these tertiary structure and theoretical methods for epitope prediction has been led to synthesis of such peptides that are important for immunodiagnostic tests and vaccines. Bioinformatic tools to better understanding and characterizing the OprF, OprL and Oprl structure of P. aeruginosa was used. For homology modeling, BLAST was run on the sequence in order to find the best template. The template was then served to model the 3D structure. Also, secondary structure of the proteins was predicted. Moreover, topology, signal peptide and B-cell epitopes of proteins were predicted.
Farnoush Farjam, Mitra Fakhar Khorasgani, Mojtaba Kiany Boroujeni, Hooriyeh Ranjbaran, Tayebeh Abdpoursogh, Navid Farahmandian and Fateme Sefid, 2017. Recombinant OprF-OprL-OprI as a Vaccine Against Pseudomonas aeruginosa Infections. Journal of Engineering and Applied Sciences, 12: 1603-1611.