Authors : F. Mariam-Aisha, Y.H. Foo, S. Neelam and M.Y. Mohd Fairuzuddin Faizan
Abstract: Lignocellulose is the most abundant renewable resource available from plants in this case from coconut husk. Bioconversion products of lignocelluloses have biomedical significance. This study was to identify previously isolated lignocellulosic bacteria strains which have the ability to degrade lignin and cellulose. The techniques used in this study confirms bacteria identity by sequence analyses of 16s rRNA, Gram staining, motility test, crystal formation test and penicillin sensitivity test (10 μg of Penicillin G-P-10). Gram staining was performed on six bacteria strains to distinguish between Gram negative and Gram positive cells. Extraction of genomic DNA for 16s rRNA sequence analyses were also conducted on the six bacteria strains to differentiate between species. Four out of six bacteria were identified as Bacillus thuringiensis (T10), Enterobacter aerogenes (T19), Bacillus pumilus (T22) and Bacillus vireti (T24) using sequence analysis of 16s rRNA compared to NCBI database. Two bacteria strains, C19 and C37 belong to the Genus Bacillus which had more than one possible species results which were distinguished by using biochemical test. Four bacteria strains were Gram positive (T10, T22, C19 and C37) and two Gram negative (T19 and T24). Motility test was negative for C19 and positive in C37 isolates. Isolate C19 had a diameter of 32 mm which showed susceptibility to Penicillin whereas for isolate C37 had a diameter of 19 mm which showed resistance to Penicillin. Isolate C37 was confirmed as B. thuringiensis after four days incubation by using crystal formation test. This study suggests that the isolates T10, T19, T22, T24, C19 and C37 were identified as B. thuringiensis, E. aerogenes, B. pumilus, B. vireti, B. anthracis and B. thuringiensis, respectively. Future studies using metabolic engineering techniques can optimise their lignocellulose degradation potential in suitable vectors.
F. Mariam-Aisha, Y.H. Foo, S. Neelam and M.Y. Mohd Fairuzuddin Faizan, 2017. 16s rRNA Identification of Lignocellulose Degrading Bacteria from Cow Dung and Termite GUT Revealed Pathogenic Bacteria Strains. Journal of Engineering and Applied Sciences, 12: 1777-1781.