Abstract: High volume of traffic at the toll gate pasteur is the main cause of the queues at the substation out, with the issue PT. Jasamarga provide GTO (Gerbang Tol Otomatis) substation built to serve transactions using e-toll card and speed up transactions at the substation out so it can reduce the queues that occur at the toll gate pasteur. To find significant use of GTO substations can reduce the queues that occur in substations exit at the toll gate cluster 1, the purpose of this study is to performance analysis to determine the level of performance of the substation GTO system and the actual maximum capacity of the volume of traffic that can be served by the use of GTO booth at the toll gate pasteur. The study began by collecting the data necessary to create simulation models, data has been collected and processed using software StatFit to determine the pattern of distribution of the data, once complete data is continued with the development of simulation models using Promodel 2014 student version. The output of the simulation model created later validated by testing t-test using SPSS software. Once the models are made valid, then the research continued with experimentation models to obtain data in accordance with the purpose of research. Data from these experiments is then analyzed to obtain a final conclusion. Performance analysis of simulation output results showed the highest utility substations GTO of 23.30% with 4.46 seconds service time at the toll gate pasteur Analysis for maximum capacity of substations GTO at toll gate pasteur can serve 102,500 vehicles a day at 8 substations operating GTO.