Abstract: The purpose of this study was to determine the characteristics of acute substance poisoning patients admitted at a regional emergency medical center located in an urban rural complex. The general characteristics of medical records, occurrence season and emergency care records of acute substance poisoning patients among patients admitted into an emergency medical center located in S region, Chungnam from January to December 2015 were evaluated. SPSS Win 22.0 Program was used for statistical analysis including Chi-square test and Fisher’s exact test.The percentage of acute substance poisoning patients in the total number of patients admitted at the emergency medical center was 0.12%. Within the intentional substance poisoning group, 70.4% were female patients. This was higher than that (63.6%) in the unintentional poisoning group. Patients were divided into the elderly group and the non-elderly group using a cutoff value of 65 years of age. The percentages of both the intentional substance poisoning group and the unintentional poisoning group were higher in the elderly group than those in the non-elderly group. As for the presence of diseases, there were more cases in the intentional poisoning group compared to that in the unintentional poisoning group. The use of 119 ambulance was the most frequent admission method for both intentional poisoning group and unintentional poisoning group. The use of other vehicles was more common in the unintentional poisoning group compared to that in the intentional poisoning group. Of different months, April was the most frequent months (18.5%) for cases of intentional poisoning while May and December were the most frequent months for cases of unintentional poisoning (27.3%). Emergency treatment results was in the order of hospitalization, transfer and recovery for the intentional poisoning group rather than unintentional poisoning group. The general characteristics and substance poisoning related characteristics of subjects were determined. These data will provide basis for suicide prevention programs and national health program.