Abstract: Austenetic stainless steel AISI 316L are frequently used as orthopedic implants because their mechanical properties and formability. In this research, a film of Hydroxyapatite (HA) was prepared by a PLD technique on 316L SS. HA was pressed at pressure (150 MPa) with partical size (2.745 μm) and used as a target in the coating. Effects of the number pulses (3000, 4500 and 6000) on the coating layer properties have been studied. Effects of the annealing temperature (450°C) for 1 h and under an empty atmosphere on the coating layer properties have been studied, also. The microstructure and composition of the coating were analyses by Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS) and X-Ray Diffraction (XRD). The mechanical property of the coating was evaluated by Vickers micro hardness test. The electrochemical corrosion behaviors and Ni ions release of uncoated and coated samples with different number of pulses in synthetic saliva and hanks solution were also, investigated. The XRD analysis of coated sample indicate the formation of the HA layer. SEM results shows evident improvement in microstructure and grow HA film with increasing in pulses number. In bioactivity test the percentage of (Ca ) and (P) are increased significantly after 14 days immersion in SBF which confirms that the samples are bioactive. A significant corrosion test results show that the corrosion rate after HA coating decreased from (2.1370*10-3 mpy) for uncoated sample into (0.05110*10-3 mpy) for coated sample in synthetic saliva. And from (9.26221*10-3 mpy ) for uncoated sample into (0.34206*10-3 mpy) for coated sample in Hanks solution. The Ni release test shows that the amount of releasing Ni decreased from (0.56716 ppm) for uncoated samples into (0.11940 ppm) for coated sample in synthetic saliva. And Ni ions decreased from (10.4179 ppm) for uncoated samples into (0.2686 ppm) for coated sample in Hanks solution and another interesting increase in biocompatibility was achieved during the research for the coated sample compare to the uncoated 316L stainless steel sample.