Authors : Peni Indrayudha, Haryoto and Kosworo
Abstract: Soursop (Annona muricata L.) has been used as traditional medicine. Soursop as Annonaceae family, contain alkaloids, acetogenins, polyphenols, terpenes and aromatic compounds. This study has the aimto determine the Minimum Inhibitory Concentration (MIC) of the leaf of soursop (Annonamuricata L.) ethanol extract polar fraction against to Klebsiella pneumoniae and Staphylococcus epidermidis and identify the active compound which responsible for the activity. The leaf of Annona was extracted using 96% ethanol by maceration and fractionation with Vacuum Liquid Chromatography (VLC). Antibacterial activity of polar fraction was tasted using solid dilutions method. Polar fraction concentration series of used was 2.5, 3, 3.5, 4 and 4.5% w/v. To determine compounds performed by Thin Layer (TLC) using silica as stationary phase and hexane: ethylacetate (3:7) as the mobilephase. Bioautography using TLC plate was placed inoculated bacteria, antibacterial compounds indicated an inhibition zone. The results showed that polar fraction of ethanol extract of leaves of the soursop has antibacterial activity against Klebsiella pneumoniae and Staphylococcus epidermidis with MIC were 3 and 3.5% w/v, respectively. Class of compounds that have antibacterial activity against Klebsiella pneumoniae and Staphylococcus epidermidis were flavonoids, polyphenol, antron and triterphenoid.
Peni Indrayudha, Haryoto and Kosworo , 2019. Antibacterial Activity and Bioautography Polar Fraction of Ethanolic Extracts of Leaves of Soursop (Annona muricata L.) Against Klebsiella pneumoniae and Staphylococcus epidermidis. Journal of Engineering and Applied Sciences, 14: 6101-6105.