Authors : Oghenerobor B. Akpor, Olarewaju M. Oluba, Olayinka O. Alabi, Abigail G. Adeyonu and Ayoola J. Shoyomb
Abstract: To reduce the production cost of growth medium for microbial cultivation, a variety of natural products such as milk, animal tissues and plants have been exploited. The present study was aimed at generating Chicken Feather Protein Hydrolysate (CFPH) from alkaline hydrolysis of chicken feathers as a medium component in the cultivation of selected bacteria and yeasts. Alkaline hydrolysis of the raw chicken feather was carried out to obtain CFPH. The study was carried out using different combinations of CFPH: peptone ratios for the growth medium. Three bacteria Esherichia coli, Staphylococcus aureus and Klebsiella pneumonia) and two yeasts (Candida carpophila and Candida tropicalis) species were as the test microbial species used as test microorganisms. For growth rate studies, 0.5 mL of the broth cultures of the respective test isolates was inoculated in the growth medium. Immediately after inoculation and at every 2 h interval, for a 10 h duration, aliquot samples of the inoculated broth cultures were aseptically withdrawn from each flask for optical density measurement. The study revealed 60 % CFPH yield with the hydrolysis method employed. The results obtained demonstrated that growth performance of the test microorganisms varied for each of the CFPH: peptone combinations. Generally, growth rates of the yeast were observed to be significantly higher (p<0.05) in media with CFPH: peptone combinations of 4:6, 6:4 and 8:2 than media with peptone only. Although, in most of the bacteria species investigated, growth was better in the peptone only media, media containing 4:6, 6:4 and 8:2 CFPH: peptone combinations compared well in terms of growth rate. Data obtained in this study showed the potential of CFPH as an alternative growth substrate to peptone in microbial culturing. Thus, revealing the possibility of conversion of chicken feather waste to more valuable use.
Oghenerobor B. Akpor, Olarewaju M. Oluba, Olayinka O. Alabi, Abigail G. Adeyonu and Ayoola J. Shoyomb, 2019. Chicken Feather Hydrolysate as Potential Peptone Source for Bacterial and Yeast Cultivation. Journal of Engineering and Applied Sciences, 14: 8721-8726.