Authors : Hendri Poernomo, Mochammad Taha Ma`ruf and Durra Mufida
Abstract: The objective of this research is to determine the application effect of mangosteen-skin (Garcinia mangostana L.) extract gel to the number of fibroblast and collagen width during wound healing process after tooth extraction of guinea pigs. In vivo experimental study consist of 24 guinea pigs divided into 4 groups. Extraction of lower right incisive performed and mangosteen-skin extract gel applied later with different concentration of 20, 40 and 60% for each group. On the 7th day, guinea pigs were decapitated and prepared using Haematoxylin Eosin (HE) and Mallory staining. The number of fibroblast cell and collagen matrix width is measured using binocular microscope. Result shows significant difference on the number of fibroblast and collagen width among experimental group (p<0.05). Mangosteen-skin extract gel with 40% concentration further increased the number of fibroblast and collagen width than the rest of the group of 20% concentration, 60% concentration and control group. It could be concluded that mangosteen-skin extract gel with 40% concentration could accelerate wound-healing process after tooth extraction of guinea pig, indicated by increasing number of fibroblast and collagen width. This condition achieved because of the mangosteen-skin containing gamma-mangosteen an active compound with anti-inflammation activity. Gamma-mangosteen blocked the transformation process of arachidonic acid to PGE on the cyclooxygenase track.
Hendri Poernomo, Mochammad Taha Ma`ruf and Durra Mufida, 2019. An Analysis of Mangosteen-skin (Garcinia mangostana L.) Extract Gel to Increase the Number of Fibroblast and Collagen Width During Wound Healing Process after Tooth Extraction of Guinea Pigs. Journal of Engineering and Applied Sciences, 14: 9310-9314.