Abstract: The approximate, protein and inorganic constituents of two economically important freshwater clariids were determined and their corresponding water quality of the habitat was conducted to investigate the environmental safety and the process of obtaining nutrition necessary for health and growth gains to consumers. The 50 fish samples of Chrysichthys nigrodigitatus (Silver catfish) and Clarias gariepinus (African Mud catfish) each with weight ranging from 500-800 g purchased at fish landing site in Ojo, Lagos Nigeria were examined for proximate, mineral and amino acid compositions determined by Atomic Absorption Spectrophotometer and Gas chromatography, alongside Association of Official Analytical Chemists (AOAC) methods, respectively. Highest values of 44.93±0.04 and 38.61±0.19 crude protein were found in them and the smallest worth of 3.96±0.02 and 3.77±0.02 ash found in C. nigrodigitatus and C. gariepinus separately. The natural oily deposits 9.38±0.03 and 10.42±0.04 were very high in both species. An important distinct (p<0.05) across Fe composition of C. gariepinus (2.18±0.04) and C. nigrodigitatus (1.14±0.16). Na (80.07±0.55) and Mg (74.55±1.00) constituents were high up for C. gariepinus. Pb and Cd differed significantly (p<0.05) along both species while Ni was not detected in both species. Tryptophan (Trp) and Cysteine (Cys) were found to be the lowest (1.66±0.05 and 0.04±0.01) and (1.51±0.05 and 1.22±0.03) for C. nigrodigitatus and C. gariepinus, respectively. Of the 18 detected amino acids, Pro (2.06±0.05 and 2.72±0.07), Thr (7.31±0.34 and 3.95±0.03), Gln (15.28±0.05 and 13.28±0.06) and Trp (1.66±0.05 and 0.04±0.01) were observed to be importantly distinct (p<0.05) for both fish species. The high fat and crude protein detected in C. nigrodigitatus and C. gariepinus was an indication that they could be good sources of fish oil and protein and as such required for growth and normal body functions. The water quality indicates good indices that both catfishes reside in safe environment and within the FEPA, WHO and EU standards for freshwater. Therefore, both species are good sources of medicinal requirements, especially for people with critical nutritional imbalance.