Abstract: Osmoregulation, which involves maintenance of cell turgor by accumulation of solutes in response to drought stress, is one of the mechanisms involved in drought tolerance. Several methods have been proposed to be used in order to differentiate wheat genotypes in terms of osmoregulation capability. Among this coleoptile growth under drought stress condition is a valuable method as cultivars comparison and hence selection could be done at early growth stages. In order to compare osmoregulation capability of wheat genotypes which are recommended for cultivation under different environmental condition in Iran, an experiment was conducted in the crop science laboratory of Shahid Bahonar university of Kerman. Seeds were germinated in dark long caps under normal and drought stress condition which was imposed by 20 and 30% polyethylene glycol (6000) solutions. Coleoptile growth was measured in nearest mm 48 hours after germinated seeds exposed to drought stress. Frequency distribution of coleoptile growth under stress condition showed a bimodal distribution to each a significant normal distribution was fitted. Suggesting that genotypes could be classified into two different groups the first distribution located at 8.93 mm and the second at 21.4 mm. Higher coleoptile growth in the first group was attributed to high osmoregulation capability, while lower growth was assigned to low osmoregulation capability. On the other hand, cultivars with high coleoptile growth had significantly higher grain yield compared to those with low coleoptile growth under drought stress condition. High osmoregulation cultivars were suggested to be used for cultivation under dry condition and in breeding programs to increase drought tolerance by.