Authors : A.A. Soro, K.M. Dje and P. Thonart
Abstract: The objective of this study was to assess changes in membrane permeability during rehydration of freeze-dried Weissella paramesenteroides LC11. Viability was assessed using the electrical conductivity measurement (ms/cm/g dry weight) and the plate count method (cfu g-1 dry weight). The symptoms of injury included an increase in the electrolyte leakage during the first 4 h of rehydration in Milli Q water and a decrease in the survival rate, suggesting an increase in membrane permeability during dehydration. An increase in the temperature, NaCl or monosodium glutamate concentration and a decrease in H+ concentration resulted in an increase in the electrolyte leakage and a decrease in the survival rate. However, a decrease in the electrolyte leakage was observed with increasing glycerol, sucrose or maltodextrin and resulted in the maintenance of cell viability. Regression analysis showed that overall, there was a good correlation between the survival rate and electrolyte leakage (R2>0.90). Change in membrane permeability might lead to electrolyte leakage during rehydration and ultimately, cell death.
A.A. Soro, K.M. Dje and P. Thonart, 2010. Changes in Membrane Permeability During Rehydration of Freeze-Dried Weissella paramesenteroides LC11. Research Journal of Applied Sciences, 5: 7-12.