Abstract: Pesticides that are purposely applied suppress plant and animal pests and to protect agricultural and industrial products. However, the majority of pesticides is not specifically targeting the pest only also affect plants and animals and leads to loss of biodiversity. This research aimed to study environmental impact and health impact form pesticide of para rubber farmers at Phon Subdistrict Kham Muang District Kalasin Province. The samples of this study were 60 farmers with stratified random sampling techniques, the soil and para rubber at Phon Subdistrict Kham Muang District Kalasin Province which had been collection areas by dividing the sample into 4 groups by village growing regions. The instrument of this research were divided in to three kinds which are the blood cholinesterase testing kit by reactive study and the GT-pesticide residual test kit for detecting the chemical in the soil sample. The results revealed that the pesticide in the soil form para rubber plantations at Phon Subdistrict Kham Muang District Kalasin Province before the participatory process showed there Methamidophos, Monocrotophos and Dicrotophos in 4 groups but after the participatory process do not showed. The farmers after the participatory process experimental group the amount of the cholinesterase level in the blood better than the control group.