Authors : Khor Goot Heah, Nur Rawaidah Bt Mohd Shobri, Nurul Ain Bt Khoruddin, Syairah Nabila Bt Suhaimi, Yusmiaidil Putera B. Mohd Yusof and Tang Thean Hock
Abstract: Assessment of cell viability plays a fundamental role in various aspects of basic and biomedical research. It provides crucial initial data prior to performing in vivo and clinical studies. Various types of assay are available for the detection of cell viability which range from the routine trypan blue dye exclusion assay to highly complex analysis of individuals cells. One of the fundamental key to decide before choosing an assay format is the information desired to be obtained at the end of a treatment period. Over the years, di Methyl Thiazoldiphenyl-Tetrazoliumbromide (MTT) based assay is one of the most exploited approaches in cancer research for measuring cell viability, cell proliferation and drug cytotoxicity. MTT assay involves conversion of MTT tetrazolium salt to purple formazon products by viable proliferating cells. MTT assay is known for its rapid and sensitive assay, thus in this review, we focus specifically on MTT-based assay which assess the viability of cell. Besides, this review also focused on the discussion of a standard procedure using MTT assay for measuring viability of cell.
Khor Goot Heah, Nur Rawaidah Bt Mohd Shobri, Nurul Ain Bt Khoruddin, Syairah Nabila Bt Suhaimi, Yusmiaidil Putera B. Mohd Yusof and Tang Thean Hock, 2017. A Review on Di Methyl Thiazoldiphenyl-Tetrazoliumbromide (MTT) Assay in Cell Viability. Research Journal of Applied Sciences, 12: 372-378.