Authors : Jun-Ming Guo , Wei-Hong Zhao and Bing-Xiu Xiao
Abstract: Survivin gene plays an important role in the growth of cancer cells. To investigate its effects on the proliferation of MGC-803 cells, the expression of survivin gene in MGC-803 cells was silenced by the siRNA expression vector-based RNA interference (RNAi) technique. The survivin siRNA expression plasmid was transfected into MGC-803 cells by lipofectamine. The expression of survivin mRNA was determined by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The changes of cell cycle distribution and the cell proliferation were analyzed using flow cytometry and MTT assay, respectively. The survivin siRNA expression plasmid significantly down-regulated the expression of survivin gene in MGC-803 cells with a percentage of 48.2% (vs vector controls) and it arrested cell at G0/G1 phase (77.4%). The cell proliferation was significantly inhibited and the optical density in siRNA-transfected cells was markedly lower than that in the vector controls (p< 0.01). The growth inhibitory rates were 53.4, 66.7 and 86.3% after 24, 48 and 72 h of the transfection, respectively. Down-regulation the expression of survivin can inhibit the cell proliferation of gastric cancer cells in vitro.
Jun-Ming Guo , Wei-Hong Zhao and Bing-Xiu Xiao , 2007. Suppression of Gastric Cancer Cell Growth by Suppressing Survivin Gene Expression Using RNAi Technology . Research Journal of Biological Sciences, 2: 687-690.