Authors : Bill E. Cham , A.D. Shannon , X. Gu and A. Elliott
Abstract: Lipid Associated Viruses (LAVs) including HIV, hepatitis B, C and SARS are amongst the most malevolent viruses. These and a variety of other viruses have an outer lipid envelope which maintains inserted viral peptides in the "correct" functional conformation and orientation. Most solvents disrupt the lipid envelope and destroy infectivity but these solvents also result in a loss of antigenicity. Specific organic solvents consisting of butanol and Diisopropyl Ether (DIPE) delipidate the whole virion rendering it non-infective, but antigenic. In large animals such as cattle, delipidation of large amounts of the pestivirus Bovine Viral Diarrhea Virus (BVDV) result in the inactivation of the virus shown by in vitro and in vivo testing. The inactivated BVDV preparation when used as a vaccine results in anti-E2 antibody production in all vaccinated animals. Delipidation of lipid-associated enveloped viruses with specific organic solvents has potential as the basis for development of vaccines.
Bill E. Cham , A.D. Shannon , X. Gu and A. Elliott , 2007. Delipidation of a Pestivirus: Viral Inactivation and Vaccine Development in Large Animals . Research Journal of Biological Sciences, 2: 706-712.