Authors : S.H. Montazam , S. Faragnia , R. Aboshof and H. Rezaee
Abstract: Penicillin G Acylase (PGA) is an important enzyme in the bulk pharmaceutical industry. It is one of the most important industrial enzymes for the production of semi-synthetic penicillin. PGAs have been found in numerous bacteria and fungi. The PGA gene has been isolated from different organism displays distinct biochemical properties which may be important for industrial aspects. In this study, 250 non E. coli, Enterobacteriasea that were collected from environmental and clinical samples, Transported to the laboratory and subjected for routine microbiological tests for identification of isolates. After identification, non E. coli isolates were investigated by PCR for processing of PGA gene. In this method, a PGA positive strain from an isolate that identified as Shigella boydii by standard microbiological test. DNA extracted from Shigella boydii entered in PCR reactions using primers designed on conserved region of PGA genes. The gene were cloned in pGEM-Teasy vector and submitted for sequencing. The gene encoding a Penicillin G acylase from Shigella boydii isolate contain an open reading frame of 2534 nucleotide encoding 796 amino acids. Analysis of sequencing result showed that this gene contain 98% homology to previously reported PGA from a Shigella boydii strains.
S.H. Montazam , S. Faragnia , R. Aboshof and H. Rezaee , 2008. Isolation, Identification and Cloning of the Penicillin G Acylase Gene from Shigella boydii Cloned in Escherichia coli. Research Journal of Biological Sciences, 3: 1182-1185.