Authors : Fatemeh Moeini Alishah , Ladan Hosseini Gohari and Leila mostean
Abstract: Accurate identification and quantification of normal and abnormal Hemoglobin (Hb) variants are clinically important for the diagnosis of hemoglobinopathies. Most of the common hemoglobinopathies can be identified by cellulose acetate electrophoresis in alkaline pH and citrate agar electrophoresis in acidic pH. For uncommon mutations, chromatographic globin chain separation and DNA sequencing are the complementary diagnostic tests. The present report concerns 20 unknown Hb variants among the patients who referred to our center. Cellulose acetate electrophoresis at alkaline pH was performed and all the variants were separated out in HbS region. On agar gel electrophoresis at pH 6.2 all of the Hb variants were separated out between HbS and HbC region. Chromatographic (CM cellulose) separation of the chains in the presence of 8 M urea showed the co-existence of alpha chain variants in 15 specimens, co-existence of beta chain variants in 3 specimens and the co-existence of both alpha and beta chain variants in the 2 remaining patients. In conclusion, globin chain separation is a helpful guideline for the selection of an appropriate gene for DNA sequencing.
Fatemeh Moeini Alishah , Ladan Hosseini Gohari and Leila mostean , 2008. Detection of Globin Chains Variants by Ion Exchange Chromatography. Research Journal of Biological Sciences, 3: 401-404.